Biochemistry biochemistry1
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ORPHEE GROUP

Biochemistry Reagent

Parameter Method Composition (R1:R2) Shelf life
SUBSTRATES      
Albumin Colorimetric, bromocresol green, end-point, 630 nm 1x5000 33
Microalbumin Immunoturbidimetric, 340 cm 1x4000 / 2x400 48
Bilirubin total II Genearation Vanadate oxidation method, end-point, 420 (450)nm 1x4169 / 2x416.9 18
Bilirubin total Maloy-Evelyn Maloy-Evelyn method, 530nm 1x4000 / 2x500 18
Bilirubin direct III Generation Vanadate oxidation method, end-point, 420 (450)nm 1x4000 / 2x500 24
Bilirubin direct Maloy-Evelyn Maloy-Evelyn method, 530nm 1x4445 / 2x278 24
Cholesterol  Enzymatic (CHOD/POD), Colorimetric, 500 cm (546 nm) 1x5000 18
Cholesterol HDL II Generation direct method based on selective inhibition, 630nm 1x3000 / 2x500 24
Cholesterol LDL II Generation direct method based on selective inhibition, 630nm 1x3000 / 2x500 24
Creatinine Colorimetric, Jaffe wothout deproteinization, kinetic, 500 nm (492 nm) 1x4000 / 2x500 36
Glucose Enzymatic(GOD/POD), colorimetric, end-point, 500nm (546 nm) 1x5000 18
Total protein II Generation Colorimetric, biuret reagent, end-point, 546nm 1x5000 36
Urine proteins Direct, colorimetric method with pyrogallol red, 600nm 1x5000 12
Triglycerides Colorimetric, Enzymatic(GOD/POD), end-point, 550nm(546nm) 1x4000 / 2x500 18
Urea Enzymatic (urease/ GLDH) , kinetic, 340nm (334nm,365nm) 1x4000 / 2x500 18
Triglycerides (monoreagent) Colorimetric, Enzymatic(GOD/POD), end-point, 550nm(546nm) 1x5000 18
Uric acid Enzymatic (uricase), colorimetric, end-point, 546nm (530-550nm) without ascorbate oxidase (AOX) 1x4000 / 2x500 18
Creatinine (enzymatic) Enzymatic (creatininase), Colorimetric , end-point, 546nm 1x3000 / 2x500 18
Cholesterol HDL Precipitation with phosphotungstic acid, 500 nm(546nm) 1x5000 28
Bile acids Enzymatic(3-a-HSD), end-point, 405nm 1x1500 / 1x500 12
Glucose HEX Colorimetric, enzymatic (lactate oxidase, POD) 546nm 1x5000 / 2x500 36
Lactate Colorimetric, enzymatic (lactate oxidase, POD) 546nm 1x1500 24
Enzymes      
Amylase CNP-G3, MES buffer, kinetic, 405nm 1x5000 27
Amylase CNP-G3, MES buffer, kinetic, 405nm 1x4000 / 2x500 36
ALT (GPT) IFCC modified, TRIS buffer, without pyridoxal phosphate, kinetic, 340nm(334nm,365 nm) 1x4000 / 2x500 18
AST (GOT) IFCC modified, TRIS buffer, without pyridoxal phosphate, kinetic, 340nm(334nm,365 nm) 1x4000 / 2x500 18
ALP IFCC method, AMP buffer, kinetic, 405nm 1x4000 / 2x500 24
Creatine kinase (CK) CK NAC, IFCC method, kinetic, 340nm (334,365 nm) 1x5000 / 2x500 15
CK-MB CK NAC, IFCC method, kinetic, 340nm (334,365 nm) 1x5000 / 2x500 15
GGT SZASZ、TRIS buffer, kinetic, 405nm 1x4000 / 2x500 18
LDH DGKC method, kinetic, 340nm (334,365 nm) 1x4000 / 2x500 18
Cholinesterase DGKC kinetic, 405 nm 1x4000 / 2x500 18
α-HBDH DGKC method, kinetic, 340nm (334,365 nm) 1x4000 / 2x500 18
ELECTROLITES      
Calcium Colorimetric, o-cresolphthalein complexone, end-point, 575nm (570-580nm) 1x4000 / 2x500 30
Phosphorus Phosphomolybdate, end-point, 340nm (334,365 nm) 1x5000 18
Magnesium Colorimetric, xylidyl blue, end-point, 520nm (500nm,546 nm) 1x5000 12
Iron Colorimetric with ferrozine, without deproteinization, end-point, 550nm (560-570nm) 1x4170 / 2x417 15
Calcium (Arsenazo III) Colorimetric, Arsenazo III, end-point, 650nm,623nm (630-670 nm) 1x5000 36
UIBC Colorimetric with ferene, end-point, 578nm (570-600nm) 1x4000 / 2x500 12
IBC Saturation solution 1- Sat. Sol. 2x400 ml / 2x Magnes. Hyd. 18
dTIBC Colorimetric, Direct, with chromazurol B, 100, 660nm 1x4000 / 2x500 12
SPECIFIC PROTEINS      
HbA1c Immunoturbidimetric, 670 nm 1x3750 + 1x1250 +hemolytic reagent + 1x 9400 ml 24
D-Dimer Immunoturbidimetric, 670 nm 1x3750 + 1x 625 + d-dimer diluent + 2x 2500 18
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